PoplarV1, Main, Exploration, bibRecord, 001408

Enhanced delignification of steam-pretreated poplar by a bacterial laccase.

Identifieur interne : 001408 ( Main/Exploration ); précédent : 001407; suivant : 001409

Enhanced delignification of steam-pretreated poplar by a bacterial laccase.

Auteurs : Rahul Singh [Canada] ; Jinguang Hu [Canada] ; Matthew R. Regner [États-Unis] ; James W. Round [Canada] ; John Ralph [États-Unis] ; John N. Saddler [Canada] ; Lindsay D. Eltis [Canada]

Source :

Scientific reports [ 2045-2322 ] ; 2017.

RBID : pubmed:28169340

Descripteurs français

KwdFr :
- Actinobacteria (composition chimique), Actinobacteria (enzymologie), Biocarburants (ressources et distribution), Biocatalyse (MeSH), Biomasse (MeSH), Cellulase (composition chimique), Cinétique (MeSH), Humains (MeSH), Laccase (composition chimique), Laccase (isolement et purification), Lignine (composition chimique), Populus (composition chimique), Protéines bactériennes (composition chimique), Protéines bactériennes (isolement et purification), Spécificité du substrat (MeSH), Vapeur (MeSH).
MESH :
- composition chimique : Actinobacteria, Cellulase, Laccase, Lignine, Populus, Protéines bactériennes.
- enzymologie : Actinobacteria.
- isolement et purification : Laccase, Protéines bactériennes.
- ressources et distribution : Biocarburants.
- Biocatalyse, Biomasse, Cinétique, Humains, Spécificité du substrat, Vapeur.

English descriptors

KwdEn :
- Actinobacteria (chemistry), Actinobacteria (enzymology), Bacterial Proteins (chemistry), Bacterial Proteins (isolation & purification), Biocatalysis (MeSH), Biofuels (supply & distribution), Biomass (MeSH), Cellulase (chemistry), Humans (MeSH), Kinetics (MeSH), Laccase (chemistry), Laccase (isolation & purification), Lignin (chemistry), Populus (chemistry), Steam (MeSH), Substrate Specificity (MeSH).
MESH :
- chemical , chemistry : Bacterial Proteins, Cellulase, Laccase, Lignin.
- chemistry : Actinobacteria, Populus.
- enzymology : Actinobacteria.
- chemical , isolation & purification : Bacterial Proteins, Laccase.
- chemical , supply & distribution : Biofuels.
- Biocatalysis, Biomass, Humans, Kinetics, Steam, Substrate Specificity.

Abstract

The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several bacterial ligninases promises the development of novel biocatalysts, these enzymes have largely been characterized using model substrates: direct evidence for their action on biomass is lacking. Herein, we report the delignification of woody biomass by a small laccase (sLac) from Amycolatopsis sp. 75iv3. Incubation of steam-pretreated poplar (SPP) with sLac enhanced the release of acid-precipitable polymeric lignin (APPL) by ~6-fold, and reduced the amount of acid-soluble lignin by ~15%. NMR spectrometry revealed that the APPL was significantly syringyl-enriched relative to the original material (~16:1 vs. ~3:1), and that sLac preferentially oxidized syringyl units and altered interunit linkage distributions. sLac's substrate preference among monoaryls was also consistent with this observation. In addition, sLac treatment reduced the molar mass of the APPL by over 50%, as determined by gel-permeation chromatography coupled with multi-angle light scattering. Finally, sLac acted synergistically with a commercial cellulase cocktail to increase glucose production from SPP ~8%. Overall, this study establishes the lignolytic activity of sLac on woody biomass and highlights the biocatalytic potential of bacterial enzymes.

DOI: 10.1038/srep42121
PubMed: 28169340
PubMed Central: PMC5294454

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 001451
to stream Main, to step Curation: 001451

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Enhanced delignification of steam-pretreated poplar by a bacterial laccase.</title>
<author><name sortKey="Singh, Rahul" sort="Singh, Rahul" uniqKey="Singh R" first="Rahul" last="Singh">Rahul Singh</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</nlm:affiliation>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<country>Canada</country>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hu, Jinguang" sort="Hu, Jinguang" uniqKey="Hu J" first="Jinguang" last="Hu">Jinguang Hu</name>
<affiliation wicri:level="4"><nlm:affiliation>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4</wicri:regionArea>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Regner, Matthew R" sort="Regner, Matthew R" uniqKey="Regner M" first="Matthew R" last="Regner">Matthew R. Regner</name>
<affiliation wicri:level="2"><nlm:affiliation>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726</wicri:regionArea>
<placeName><region type="state">Wisconsin</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry, University of Wisconsin, Madison, WI 53706</wicri:regionArea>
<placeName><region type="state">Wisconsin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Round, James W" sort="Round, James W" uniqKey="Round J" first="James W" last="Round">James W. Round</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</nlm:affiliation>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<country>Canada</country>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
<affiliation wicri:level="2"><nlm:affiliation>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726</wicri:regionArea>
<placeName><region type="state">Wisconsin</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry, University of Wisconsin, Madison, WI 53706</wicri:regionArea>
<placeName><region type="state">Wisconsin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Saddler, John N" sort="Saddler, John N" uniqKey="Saddler J" first="John N" last="Saddler">John N. Saddler</name>
<affiliation wicri:level="4"><nlm:affiliation>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4</wicri:regionArea>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Eltis, Lindsay D" sort="Eltis, Lindsay D" uniqKey="Eltis L" first="Lindsay D" last="Eltis">Lindsay D. Eltis</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</nlm:affiliation>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<country>Canada</country>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, BC, V6T 1Z3</wicri:regionArea>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:28169340</idno>
<idno type="pmid">28169340</idno>
<idno type="doi">10.1038/srep42121</idno>
<idno type="pmc">PMC5294454</idno>
<idno type="wicri:Area/Main/Corpus">001451</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001451</idno>
<idno type="wicri:Area/Main/Curation">001451</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001451</idno>
<idno type="wicri:Area/Main/Exploration">001451</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Enhanced delignification of steam-pretreated poplar by a bacterial laccase.</title>
<author><name sortKey="Singh, Rahul" sort="Singh, Rahul" uniqKey="Singh R" first="Rahul" last="Singh">Rahul Singh</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</nlm:affiliation>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<country>Canada</country>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hu, Jinguang" sort="Hu, Jinguang" uniqKey="Hu J" first="Jinguang" last="Hu">Jinguang Hu</name>
<affiliation wicri:level="4"><nlm:affiliation>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4</wicri:regionArea>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Regner, Matthew R" sort="Regner, Matthew R" uniqKey="Regner M" first="Matthew R" last="Regner">Matthew R. Regner</name>
<affiliation wicri:level="2"><nlm:affiliation>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726</wicri:regionArea>
<placeName><region type="state">Wisconsin</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry, University of Wisconsin, Madison, WI 53706</wicri:regionArea>
<placeName><region type="state">Wisconsin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Round, James W" sort="Round, James W" uniqKey="Round J" first="James W" last="Round">James W. Round</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</nlm:affiliation>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<country>Canada</country>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
<affiliation wicri:level="2"><nlm:affiliation>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726</wicri:regionArea>
<placeName><region type="state">Wisconsin</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry, University of Wisconsin, Madison, WI 53706</wicri:regionArea>
<placeName><region type="state">Wisconsin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Saddler, John N" sort="Saddler, John N" uniqKey="Saddler J" first="John N" last="Saddler">John N. Saddler</name>
<affiliation wicri:level="4"><nlm:affiliation>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4</wicri:regionArea>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Eltis, Lindsay D" sort="Eltis, Lindsay D" uniqKey="Eltis L" first="Lindsay D" last="Eltis">Lindsay D. Eltis</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</nlm:affiliation>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<country>Canada</country>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, BC, V6T 1Z3</wicri:regionArea>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actinobacteria (chemistry)</term>
<term>Actinobacteria (enzymology)</term>
<term>Bacterial Proteins (chemistry)</term>
<term>Bacterial Proteins (isolation & purification)</term>
<term>Biocatalysis (MeSH)</term>
<term>Biofuels (supply & distribution)</term>
<term>Biomass (MeSH)</term>
<term>Cellulase (chemistry)</term>
<term>Humans (MeSH)</term>
<term>Kinetics (MeSH)</term>
<term>Laccase (chemistry)</term>
<term>Laccase (isolation & purification)</term>
<term>Lignin (chemistry)</term>
<term>Populus (chemistry)</term>
<term>Steam (MeSH)</term>
<term>Substrate Specificity (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Actinobacteria (composition chimique)</term>
<term>Actinobacteria (enzymologie)</term>
<term>Biocarburants (ressources et distribution)</term>
<term>Biocatalyse (MeSH)</term>
<term>Biomasse (MeSH)</term>
<term>Cellulase (composition chimique)</term>
<term>Cinétique (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Laccase (composition chimique)</term>
<term>Laccase (isolement et purification)</term>
<term>Lignine (composition chimique)</term>
<term>Populus (composition chimique)</term>
<term>Protéines bactériennes (composition chimique)</term>
<term>Protéines bactériennes (isolement et purification)</term>
<term>Spécificité du substrat (MeSH)</term>
<term>Vapeur (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bacterial Proteins</term>
<term>Cellulase</term>
<term>Laccase</term>
<term>Lignin</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Actinobacteria</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Actinobacteria</term>
<term>Cellulase</term>
<term>Laccase</term>
<term>Lignine</term>
<term>Populus</term>
<term>Protéines bactériennes</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Actinobacteria</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Actinobacteria</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Bacterial Proteins</term>
<term>Laccase</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Laccase</term>
<term>Protéines bactériennes</term>
</keywords>
<keywords scheme="MESH" qualifier="ressources et distribution" xml:lang="fr"><term>Biocarburants</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="supply & distribution" xml:lang="en"><term>Biofuels</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Biocatalysis</term>
<term>Biomass</term>
<term>Humans</term>
<term>Kinetics</term>
<term>Steam</term>
<term>Substrate Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Biocatalyse</term>
<term>Biomasse</term>
<term>Cinétique</term>
<term>Humains</term>
<term>Spécificité du substrat</term>
<term>Vapeur</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several bacterial ligninases promises the development of novel biocatalysts, these enzymes have largely been characterized using model substrates: direct evidence for their action on biomass is lacking. Herein, we report the delignification of woody biomass by a small laccase (sLac) from Amycolatopsis sp. 75iv3. Incubation of steam-pretreated poplar (SPP) with sLac enhanced the release of acid-precipitable polymeric lignin (APPL) by ~6-fold, and reduced the amount of acid-soluble lignin by ~15%. NMR spectrometry revealed that the APPL was significantly syringyl-enriched relative to the original material (~16:1 vs. ~3:1), and that sLac preferentially oxidized syringyl units and altered interunit linkage distributions. sLac's substrate preference among monoaryls was also consistent with this observation. In addition, sLac treatment reduced the molar mass of the APPL by over 50%, as determined by gel-permeation chromatography coupled with multi-angle light scattering. Finally, sLac acted synergistically with a commercial cellulase cocktail to increase glucose production from SPP ~8%. Overall, this study establishes the lignolytic activity of sLac on woody biomass and highlights the biocatalytic potential of bacterial enzymes.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28169340</PMID>
<DateCompleted><Year>2018</Year>
<Month>10</Month>
<Day>30</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>7</Volume>
<PubDate><Year>2017</Year>
<Month>02</Month>
<Day>07</Day>
</PubDate>
</JournalIssue>
<Title>Scientific reports</Title>
<ISOAbbreviation>Sci Rep</ISOAbbreviation>
</Journal>
<ArticleTitle>Enhanced delignification of steam-pretreated poplar by a bacterial laccase.</ArticleTitle>
<Pagination><MedlinePgn>42121</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/srep42121</ELocationID>
<Abstract><AbstractText>The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several bacterial ligninases promises the development of novel biocatalysts, these enzymes have largely been characterized using model substrates: direct evidence for their action on biomass is lacking. Herein, we report the delignification of woody biomass by a small laccase (sLac) from Amycolatopsis sp. 75iv3. Incubation of steam-pretreated poplar (SPP) with sLac enhanced the release of acid-precipitable polymeric lignin (APPL) by ~6-fold, and reduced the amount of acid-soluble lignin by ~15%. NMR spectrometry revealed that the APPL was significantly syringyl-enriched relative to the original material (~16:1 vs. ~3:1), and that sLac preferentially oxidized syringyl units and altered interunit linkage distributions. sLac's substrate preference among monoaryls was also consistent with this observation. In addition, sLac treatment reduced the molar mass of the APPL by over 50%, as determined by gel-permeation chromatography coupled with multi-angle light scattering. Finally, sLac acted synergistically with a commercial cellulase cocktail to increase glucose production from SPP ~8%. Overall, this study establishes the lignolytic activity of sLac on woody biomass and highlights the biocatalytic potential of bacterial enzymes.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Singh</LastName>
<ForeName>Rahul</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hu</LastName>
<ForeName>Jinguang</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Regner</LastName>
<ForeName>Matthew R</ForeName>
<Initials>MR</Initials>
<AffiliationInfo><Affiliation>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Round</LastName>
<ForeName>James W</ForeName>
<Initials>JW</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ralph</LastName>
<ForeName>John</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>US Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Saddler</LastName>
<ForeName>John N</ForeName>
<Initials>JN</Initials>
<AffiliationInfo><Affiliation>Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, The University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Eltis</LastName>
<ForeName>Lindsay D</ForeName>
<Initials>LD</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology &Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>02</Month>
<Day>07</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Sci Rep</MedlineTA>
<NlmUniqueID>101563288</NlmUniqueID>
<ISSNLinking>2045-2322</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D056804">Biofuels</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D013227">Steam</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C434469">syringyl monolignol</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9005-53-2</RegistryNumber>
<NameOfSubstance UI="D008031">Lignin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber>
<NameOfSubstance UI="D042845">Laccase</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber>
<NameOfSubstance UI="D002480">Cellulase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D039903" MajorTopicYN="N">Actinobacteria</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055162" MajorTopicYN="N">Biocatalysis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056804" MajorTopicYN="N">Biofuels</DescriptorName>
<QualifierName UI="Q000600" MajorTopicYN="N">supply & distribution</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013227" MajorTopicYN="N">Steam</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<CoiStatement>The authors declare no competing financial interests.</CoiStatement>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>09</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>01</Month>
<Day>05</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>2</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>2</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>10</Month>
<Day>31</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28169340</ArticleId>
<ArticleId IdType="pii">srep42121</ArticleId>
<ArticleId IdType="doi">10.1038/srep42121</ArticleId>
<ArticleId IdType="pmc">PMC5294454</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Syst Appl Microbiol. 2014 Feb;37(1):60-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24238986</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Chem Biol. 2015 Dec;29:18-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26231123</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Molecules. 2007 Oct 24;12(10):2327-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17978760</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomacromolecules. 2008 Dec;9(12):3362-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18991458</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2015 Dec 14;10 (12 ):e0145166</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26660105</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Recent Pat Biotechnol. 2008;2(1):10-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19075849</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Agric Food Chem. 2008 Feb 27;56(4):1398-407</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18205305</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioresour Technol. 2009 Sep;100(18):4203-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19386492</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Chem Biomol Eng. 2011;2:121-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22432613</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10143-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24982175</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Org Biomol Chem. 2010 Feb 7;8(3):576-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20090974</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochimie. 2015 May;112:151-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25778839</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Database (Oxford). 2011 Apr 15;2011:bar006</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21498547</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Biochem Biotechnol. 2009 May;157(2):174-209</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18581264</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2014 Dec;80(24):7536-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25261514</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>ACS Chem Biol. 2013 Apr 19;8(4):700-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23305326</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2011 Apr 29;6(4):e19306</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21559391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Adv. 2012 Nov-Dec;30(6):1447-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22433674</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Biotechnol. 2014 Jun;27:38-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24863895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Sci. 2004 Sep;13(9):2388-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15295117</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biotechnol. 2000 Aug 25;81(2-3):179-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10989177</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Mol Life Sci. 2015 Mar;72(5):869-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25572295</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Prog. 2009 Mar-Apr;25(2):315-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19266561</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2014 Jun 24;53(24):4047-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24870309</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Bioeng. 2010 Apr 1;105(5):871-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19998278</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioresour Technol. 2014 Oct;169:742-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25115598</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Chem Biol. 2014 Apr;19:1-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24780273</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>ACS Chem Biol. 2012 Dec 21;7(12):2074-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23054399</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Chem Soc. 2013 May 1;135(17):6415-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23570328</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2008 Jan 4;319(5859):43-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18174426</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2011 Jun 14;50(23):5096-107</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21534568</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Biotechnol. 2011 Jun;22(3):394-400</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21071202</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 1995 May;61(5):1876-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7646025</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS J. 2014 Sep;281(18):4307-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24548692</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Bioeng. 2013 Jun;110(6):1616-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23297115</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2014 Dec 24;7(1):177</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25648942</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2013 Aug 03;6(1):112</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23915398</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 1984 Oct;48(4):849-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16346649</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2005 Apr;71(4):1775-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15812000</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2012 Nov 23;338(6110):1055-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23180856</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Biochem Biotechnol. 2008 Apr;149(1):23-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18350384</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2011 Feb 10;4:3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21310050</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2012 Sep;7(9):1579-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22864199</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Canada</li>
<li>États-Unis</li>
</country>
<region><li>Colombie-Britannique </li>
<li>Wisconsin</li>
</region>
<settlement><li>Vancouver</li>
</settlement>
<orgName><li>Université de la Colombie-Britannique</li>
</orgName>
</list>
<tree><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Singh, Rahul" sort="Singh, Rahul" uniqKey="Singh R" first="Rahul" last="Singh">Rahul Singh</name>
</region>
<name sortKey="Eltis, Lindsay D" sort="Eltis, Lindsay D" uniqKey="Eltis L" first="Lindsay D" last="Eltis">Lindsay D. Eltis</name>
<name sortKey="Eltis, Lindsay D" sort="Eltis, Lindsay D" uniqKey="Eltis L" first="Lindsay D" last="Eltis">Lindsay D. Eltis</name>
<name sortKey="Hu, Jinguang" sort="Hu, Jinguang" uniqKey="Hu J" first="Jinguang" last="Hu">Jinguang Hu</name>
<name sortKey="Round, James W" sort="Round, James W" uniqKey="Round J" first="James W" last="Round">James W. Round</name>
<name sortKey="Saddler, John N" sort="Saddler, John N" uniqKey="Saddler J" first="John N" last="Saddler">John N. Saddler</name>
</country>
<country name="États-Unis"><region name="Wisconsin"><name sortKey="Regner, Matthew R" sort="Regner, Matthew R" uniqKey="Regner M" first="Matthew R" last="Regner">Matthew R. Regner</name>
</region>
<name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
<name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
<name sortKey="Regner, Matthew R" sort="Regner, Matthew R" uniqKey="Regner M" first="Matthew R" last="Regner">Matthew R. Regner</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001408 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001408 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:28169340
   |texte=   Enhanced delignification of steam-pretreated poplar by a bacterial laccase.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:28169340" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

Enhanced delignification of steam-pretreated poplar by a bacterial laccase.

Enhanced delignification of steam-pretreated poplar by a bacterial laccase.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki